Millipore Luminata Classico Western HRP Substrate User Manual

Luminata Crescendo Western HRP Substrate is a premixed, ready-to-use reagent for chemiluminescent detection in western blotting applications that employ horseradish peroxidase (HRP)- conjugated antibodies. Luminata Crescendo Substrate is ideal for the detection of medium to low abundance proteins. It can be used with traditional immunodetection techniques or the SNAP i.d.® Protein Detection System. Visualization of target proteins is achieved through x-ray film or another imaging method.


Millipore Luminata Classico Western HRP Substrate User Manual - cover page
Brand
Millipore
Document type
User Manual
Language
English
Pages
2
File format
PDF
File size
255 KB
Published
14 February, 2012
Updated
29 September, 2023
MD5 checksum
6F994786749607853CAD39F886735C34

About this manual

The Luminata™ Classico Western HRP Substrate is a premixed, ready‑to‑use chemiluminescent reagent for detecting high to medium abundance proteins in western blot applications. It can be used with traditional immunodetection methods or the SNAP i.d.® Protein Detection System, and results are visualized on x‑ray film or other imaging devices.

The manual provides package details, storage guidelines, usage instructions, troubleshooting tips, ordering information, and technical assistance contacts. It also includes performance comparisons with other Millipore substrates and safety considerations for handling the reagent.

What's inside

  • Introduction
  • Package Contents
  • Storage / Shelf Life
  • Usage Guidelines
  • Other Important Considerations
  • Chemiluminescent Detection
  • Troubleshooting
  • Ordering Information
  • Technical Assistance
  • Standard Warranty

Specifications

Storage temperature2‑8 °C
Coverage (100 mL)1,000 cm² membrane area
Coverage (500 mL)5,000 cm² membrane area
Package size (100 mL)Luminata Classico Western HRP Substrate, 100 mL
Package size (500 mL)Luminata Classico Western HRP Substrate, 500 mL
Catalogue number (100 mL)WBLUCO100
Catalogue number (500 mL)WBLUCO500

Frequently asked questions

How should Luminata Classico Substrate be stored?

Store the reagent at 2‑8 °C; protection from light is not required.

What are the catalogue numbers for the 100 mL and 500 mL bottles?

The 100 mL bottle is catalogue number WBLUCO100 and the 500 mL bottle is WBLUCO500.

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