This assay is a sandwich ELISA method for the quantitative determination of SCT in serum, plasma or other biological fluids
This manual provides detailed procedures for the Salmon Calcitonin (SCT) ELISA kit, covering sample collection, storage, reagent preparation, plate washing, and data analysis. It also includes safety notes and guidelines for optimal assay performance.
The kit uses a 96‑well plate format with standard curves ranging from 0.001 to 1.000 pg/mL and a detection wavelength of 450 nm (±10 nm). Limits of detection, specificity, and typical data interpretation are described to help users obtain reliable results. This manual is written in Chinese.
| Detection wavelength | 450 nm ±10 nm |
|---|---|
| Limit of detection | 3.9 pg/mL |
| Plate format | 96‑well plate |
| Sample types | Serum, plasma, other biological fluids |
The assay is read at 450 nm with a 10 nm bandwidth filter.
The minimum detectable concentration is 3.9 pg/mL.
The kit uses a standard 96‑well microtiter plate.