This document is an instruction manual for the Rat Vitamin K1 Enzyme-Linked Immunosorbent Assay (ELISA) Kit. The kit is designed for quantitative measurement of Vitamin K1 levels in rat serum, plasma, cell culture supernatant, or other related liquids using the ELISA method. The kit utilizes a sandwich enzyme-linked immunosorbent assay to measure Vitamin K1 levels in samples. It provides a convenient and fast way to determine the concentration of Vitamin K1 in samples.
The VK1 rat Vitamin K1 ELISA kit is designed for quantitative measurement of Vitamin K1 in rat serum, plasma, cell culture supernatants, and other related liquids using a double‑antibody sandwich ELISA format. The kit includes coated microplates, standards, sample diluents, detection reagents, substrate, and stop solution.
The manual details sample collection and storage, step‑by‑step assay procedures, data calculation methods, and important precautions such as thorough washing, avoiding light exposure, and proper reagent storage. It also notes that the English version takes precedence over the Chinese translation. This manual is written in Chinese.
| Microplate format | 96‑well plate |
|---|---|
| Standard concentration range | 10 ng/mL to 0 ng/mL (serial dilutions) |
| Detection wavelength | 450 nm |
| Reagent storage temperature | -20 °C for some reagents; 2‑8 °C for others |
| Shelf life | 6 months |
The kit can quantify Vitamin K1 in rat serum, plasma, cell culture supernatants, and other related liquids.
Measure the absorbance at 450 nm using a microplate reader.
Some reagents should be stored at –20 °C, while others should be kept at 2‑8 °C, as indicated on the label.
The kit is valid for six months from the date of manufacture.