Rat TRACP 5b Assay Kit（ELISA） is used to quantitatively determine TRAP5b in rat serum.
The RatTRAP 5b Assay Kit (ELISA) is designed for quantitative measurement of tartrate‑resistant acid phosphatase 5b in rat serum. The kit includes calibrated standards, control reagents, antibodies, substrate, and all necessary buffers for a two‑step sandwich ELISA.
The manual provides detailed steps for reagent reconstitution, plate preparation, incubation, washing, substrate development, and reading at 405 nm, as well as guidance on sample handling, storage conditions, quality‑control procedures, and result calculation.
| Samples per kit | 96 |
|---|---|
| Sensitivity | 0.1 U/L |
| Reagent storage temperature | 2‑8 °C |
| Sample type | Serum |
| Long‑term sample storage | -80 °C |
Serum samples are required; they should be collected and centrifuged immediately.
All reagents should be stored at 2 °C to 8 °C.
The assay sensitivity is 0.1 U/L.
Each kit provides enough reagents for 96 samples.