This enzyme-linked immunosorbent assay kit uses a double antibody sandwich ELISA to quantitatively measure HS levels in rat serum, plasma, tissue homogenates or other related biological fluids.
This manual provides detailed procedures for the rat Heparan sulfate (HS) ELISA kit (product number E0161Ra). It covers kit components, sample preparation, assay principle, and step‑by‑step instructions for plate handling, reagent mixing, incubation, washing, and detection.
Safety precautions, storage conditions, and troubleshooting tips are also included to ensure reliable quantitative measurement of HS in serum, plasma, or other biological samples. This manual is written in Japanese.
| Detection Range | 1.56 ng/mL – 100 ng/mL |
|---|---|
| Limit of Detection (LOD) | 0.042 ng/mL |
| Measurement Wavelength | 450 ± 10 nm |
| Plate Format | 96‑well microplate |
| Product Number | E0161Ra |
The kit can quantify HS concentrations from 1.56 ng/mL up to 100 ng/mL.
Read the absorbance at 450 nm with a ±10 nm bandwidth.
Store the reagents, standard solutions, and the 96‑well plate at –20 °C; keep the plate sealed and avoid moisture.
The product number is E0161Ra.