This kit uses a double antibody sandwich ELISA method to quantitatively measure CD40L content in rat serum, plasma, tissue homogenate or other related biological fluids.
This manual provides complete instructions for using the USCNK rat CD40 Ligand (CD40L) ELISA kit (model E0119Ra). It includes a list of supplied reagents, required equipment, sample preparation, assay principle, step‑by‑step protocol, washing procedures, and data analysis methods.
Safety notes, storage conditions, and shelf‑life information are also covered, ensuring reliable performance and accurate quantification of CD40L in serum, plasma, or other biological samples. This manual is written in Chinese.
| Detection range | 8.000 pg/mL – 125 pg/mL |
|---|---|
| Lowest detection limit | 34 pg/mL |
| Measurement wavelength | 450 nm ±10 nm |
| Shelf life | 6 months |
| Storage temperature | -20 °C (short‑term) or -80 °C (long‑term) |
The kit can quantify CD40L from 8.000 pg/mL up to 125 pg/mL.
The lowest detectable concentration is 34 pg/mL.
Read the plate at 450 nm (±10 nm).
The kit has a shelf life of six months when stored properly.