This document describes the instructions for use and expected applications of the rat urokinase-type plasminogen activator receptor (uPAR; CD87) enzyme-linked immunosorbent assay (ELISA) kit produced by Uscn Life Science Inc. Wuhan. The kit is intended for in vitro research use and quantitatively measures the uPAR content in rat serum, plasma, cell culture supernatant, or other related biological fluids.
The USCNK E0141Ra ELISA kit is designed for quantitative detection of rat uPAR (CD87) in serum, plasma, or cell culture supernatants. The kit includes a 96‑well microplate, coated antibodies, standards, detection reagents, TMB substrate, stop solution, and required buffers.
The manual describes the assay principle, sample preparation, reagent handling, incubation steps (typically 37 °C for 1–2 h), washing procedures, and how to read the results using a 450 ± 10 nm absorbance filter. Storage conditions, safety warnings, and data analysis guidance are also provided. This manual is written in Japanese.
| Kit model number | E0141Ra |
|---|---|
| Sensitivity (lowest standard) | 1.000 pg/mL |
| Reading wavelength | 450 ± 10 nm |
| Recommended storage temperature | -20 °C |
| Incubation temperature | 37 °C |
All reagents, including the standard solutions and detection buffers, should be stored at –20 °C and protected from moisture.
The absorbance is measured at 450 nm with a ±10 nm bandwidth.
The assay uses a standard 96‑well microplate.