This kit is used to detect IGFBP3 in rat serum, plasma or other related biological fluids.
This manual provides detailed instructions for the LUscn IGFBP3 ELISA kit (product number E0054Ra, 96‑well format). It covers reagent contents, required equipment, storage conditions, assay principle, sample handling, reagent preparation, step‑by‑step assay workflow, washing procedures, and data analysis.
The kit is intended for quantitative measurement of rat IGFBP3 in serum, plasma, or other biological fluids using a double‑antibody sandwich ELISA. Proper storage, handling, and adherence to the protocol are essential for reliable results. This manual is written in Chinese.
| Product number | E0054Ra |
|---|---|
| Detection range | 0.312 ng/mL – 20 ng/mL |
| Minimum detection limit | 0.087 ng/mL |
| Recommended sample dilution | ≈100‑fold |
| Reagent storage temperature | -20 °C |
| Shelf life | 6 months |
The assay can quantitatively detect IGFBP3 concentrations from 0.312 ng/mL up to 20 ng/mL.