This document provides instructions for using the Rat MAPT Enzyme-Linked Immunosorbent Assay Kit produced by Uscn Life Science Inc. It also includes information on expected applications and experimental principles. The kit is intended for in vitro research use only and is not for clinical diagnosis.
This manual provides detailed instructions for the USCNK E1983Ra rat MAPT ELISA kit. It lists all required reagents, plate format, and storage conditions, and explains the ELISA principle based on MAPT antibody binding and color development with TMB.
The document guides users through sample preparation, plate loading, incubation, washing, detection, and data analysis, and includes safety warnings for handling sulfuric acid and other reagents. This manual is written in Chinese.
| Plate format | 96-well plate |
|---|---|
| Detection wavelength | 450 nm |
| Sensitivity (lower limit) | 3.6 pg/mL |
| Shelf life after opening | 6 months |
Read the plate at 450 nm.
Store the sealed plate at –20 °C with a desiccant to avoid moisture.
The assay can detect MAPT down to 3.6 pg/mL.
The stop solution contains sulfuric acid; avoid contact with skin, eyes, and clothing.