This kit is used for quantitative measurement of ANP in rat serum, plasma or other related biological fluids.
About this manual
This manual provides detailed procedures for the USCNK E0225Ra rat atrial natriuretic peptide (ANP) ELISA kit. It covers reagent composition, storage conditions, sample collection, and step‑by‑step assay execution using a 96‑well plate.
Guidelines for plate washing, detection, data calculation, and troubleshooting are included, along with specifications such as detection range and minimum detection limit. Proper handling and storage of reagents are emphasized to ensure reliable results. This manual is written in Chinese.
What's inside
- Principle of the ANP ELISA
- Reagents and Storage Conditions
- Sample Collection and Preservation
- Assay Procedure (Plate Setup, Incubation, Detection)
- Plate Washing Methods
- Data Analysis and Curve Fitting
- Detection Limits and Sensitivity
- Safety and Waste Disposal
Specifications
| Detection range | 12.5 pg/mL – 800 pg/mL |
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| Minimum detection limit | 3.2 pg/mL |
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| Plate type | 96‑well microtiter plate |
Frequently asked questions
What is the detection range of the ANP ELISA kit?
The kit can quantify ANP concentrations from 12.5 pg/mL up to 800 pg/mL.