Rat aldehyde dehydrogenase (ALDH) enzyme-linked immunosorbent assay kit instruction manual. This kit is for research use only. It is expected to apply ELISA to quantitatively determine the content of rat serum, plasma or other related liquids by aldehyde dehydrogenase (ALDH).
The kit uses a double‑antibody sandwich ELISA format on a 96‑well plate. Reagents include standard solutions, sample diluent, conjugate, substrate (TMB) and stop solution. The protocol describes preparation of standards, sample handling, incubation steps, washing, and colour development.
Measurements are taken at 450 nm after stopping the reaction. The manual also outlines storage conditions for reagents, precautions to avoid contamination, and guidelines for data analysis and calculation of concentrations. This manual is written in Chinese.
| Detection range | 1.56 ng/ml – 100 ng/ml |
|---|---|
| Plate type | 96‑well microtiter plate |
| Measurement wavelength | 450 nm |
The assay can quantify acetaldehyde dehydrogenase concentrations from 1.56 ng/ml up to 100 ng/ml.
Absorbance should be measured at 450 nm after adding the stop solution.
Dilute the stock standard (100 ng/ml) with diluent to obtain a series of concentrations (100, 30, 23, 12.5, 6.25, 3.12, 1.56 ng/ml) and a zero‑standard.