This kit is used to quantitatively measure the level of thioredoxin (Trx) in rat serum, plasma, cell culture supernatant or other related liquids.
This manual describes the ELISA method for quantifying rat thioredoxin (Trx) in serum, cell culture supernatant, or lysates. It includes reagent preparation, sample handling, plate setup, incubation steps, and detection using TMB substrate.
The document also provides guidance on plate washing, calculation of concentrations from optical density readings, and storage conditions for reagents and samples to ensure reliable results. This manual is written in Chinese.
| Detection range | 1.56 ng/mL – 100 ng/mL |
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The kit can detect Thioredoxin concentrations from 1.56 ng/mL up to 100 ng/mL.
Store reagents at -20 °C or 2–8 °C as indicated; avoid repeated freeze‑thaw cycles and protect from light.