This document provides the instructions for use and experimental principles of the rat Müllerian inhibiting substance/anti-Müllerian hormone (MIS/AMH) enzyme-linked immunosorbent assay (ELISA) kit. The kit is designed for quantitative measurement of the MIS/AMH content in rat serum, plasma, or other related fluids using the ELISA method.
This manual provides detailed procedures for the MIS/AMH ELISA kit, including how to prepare standard and sample dilutions, coat the 96‑well plate with capture antibody, add antigen, detection antibody, HRP conjugate, and substrate. It also describes incubation times, washing steps, and the use of TMB substrate to develop color.
Guidelines for sample handling, storage conditions, and precautions to avoid contamination or assay variability are included. The document explains how to read absorbance at 430 nm, generate a standard curve, and calculate the concentration of MIS/AMH in unknown samples. This manual is written in Chinese.
| Detection wavelength | 430 nm |
|---|---|
| Standard concentration range | 0 – 20,000 pg/ml |
The absorbance is measured at 430 nm.
After each incubation, wash the plate several times with wash buffer, ensuring no bubbles remain, and tap dry before the next step.