This document provides instructions for using the Rat Osteoclast Differentiation Factor (ODF) ELISA Kit, including information on the kit components, reagent preparation, sample collection and storage, and the experimental procedure.
This manual provides step‑by‑step instructions for preparing standards, samples, and reagents, loading a 96‑well plate, and performing the ELISA assay for rat osteoclast differentiation factor (ODF). It includes details on reagent concentrations, incubation times, and washing procedures to ensure reliable measurements.
Additional sections cover plate washing methods, calculation of ODF concentrations from absorbance readings at 450 nm, storage conditions for reagents and samples, and safety precautions to avoid contamination and maintain assay accuracy. This manual is written in Chinese.
| Plate format | 96‑well |
|---|---|
| Measurement wavelength | 450 nm |
| Incubation temperature | 37 °C |
Read the absorbance at 450 nm.
The plate has 96 wells.
Incubate the plate at 37 °C.