This kit is a sandwich ELISA for detecting estrogen receptor levels in the sample.
The kit uses a double‑antibody sandwich ELISA method. Purified anti‑ER antibodies are coated on a 96‑well plate, then samples, biotinylated detection antibodies and HRP‑streptavidin are added sequentially. After washing, TMB substrate produces a color change that is measured at 450 nm.
The manual details kit components, sample collection and storage, reagent preparation, the complete assay procedure, washing methods, data analysis, and important precautions to ensure accurate results. This manual is written in Chinese.
| Detection range | 312 pg/mL – 20000 pg/mL |
|---|---|
| Standard concentration (stock) | 20000 pg/mL |
| Incubation temperature | 37 °C |
| Incubation time (first step) | 120 minutes |
| Wavelength for measurement | 450 nm |
The kit can detect estrogen receptor concentrations from 312 pg/mL up to 20 000 pg/mL.
Serum, plasma or cell‑culture supernatants should be stored at –20 °C and avoid repeated freeze‑thaw cycles.
Absorbance is measured at 450 nm using a microplate reader.
All incubation steps are performed at 37 °C.