This document describes the instructions for using the Rat Pregnancy-associated Plasma Protein A (PAPP-A) enzyme-linked immunosorbent assay (ELISA) kit, including the composition of the kit, the experimental principle, specimen collection and storage methods, and the operating steps.
This manual describes the principle, reagents, and step‑by‑step procedure for the PAPP‑A ELISA immunoassay kit. It includes preparation of standards, sample handling, plate incubation, washing, and color development using TMB substrate.
Guidelines for plate washing, data analysis, and storage of reagents and samples are provided, along with safety and precaution notes to ensure reliable results. This manual is written in Chinese.
| Reading wavelength | 450 nm |
|---|
The absorbance is measured at 450 nm.