This document is the instruction manual for the Rat Type I Procollagen (PCⅠ) ELISA Kit, which is used for quantitative measurement of the content of PCⅠ in rat tissue homogenates, serum, plasma, cell culture supernatants, or other related fluids. The experimental principle involves coating the microplate with purified antibodies to create a solid-phase carrier, and sequentially adding the specimen or standard, biotinylated anti-rat PCⅠ antibody, and HRP-labeled streptavidin. After thorough washing, the substrate TMB is used for color development. The color intensity is positively correlated with the PCⅠ content in the sample. The absorbance (OD value) is measured at a wavelength of 450nm using an ELISA reader to calculate the sample concentration.
This manual describes the intended applications of the rat type I collagen (PC I) ELISA kit, which quantifies collagen peptide levels in rat tissue homogenates, serum, plasma, cell culture supernatants, and other related liquids. The assay uses a coated microplate, biotinylated detection antibody, HRP‑streptavidin, and TMB substrate, with absorbance read at 450 nm.
The document provides detailed reagent preparation, sample handling, step‑by‑step assay procedures, washing instructions, calculation methods, and safety/ storage guidelines to ensure reliable results. This manual is written in Chinese.
| Detection Range | 1.56 ng/ml – 100 ng/ml |
|---|---|
| Lowest Detection Limit | 0.39 ng/ml |
Some reagents are stored at –20 °C, while others should be kept at 2–8 °C, following the label instructions.
Read the optical density at 450 nm using a microplate reader.
The kit is valid for 6 months from the date of manufacture.