Rat parathyroid hormone-related protein (PTHrP) enzyme-linked immunosorbent assay kit instruction manual, only for research purposes, detection range: 3.12 pg/ml - 200 pg/ml, minimum detection limit: 0.78 pg/ml, specificity: This kit can simultaneously detect natural or recombinant rat PTHrP, and has no cross-reactivity with other related proteins. Shelf life: 6 months, expected application: ELISA quantitative measurement of PTHrP content in rat serum, plasma, cell culture supernatant or other related biological fluids.
This manual describes the components, storage conditions, and step‑by‑step procedures for the Rat PTHrP ELISA kit. It covers reagent preparation, sample collection, dilution guidelines, and the ELISA workflow from plate coating to color development and measurement.
Additional sections provide guidance on equipment requirements, washing techniques (manual and automated), calculation of concentrations from standard curves, and important safety and quality notes to ensure reliable results. This manual is written in Chinese.
| Detection range | 3.12 pg/ml – 200 pg/ml |
|---|---|
| Lower detection limit | 0.78 pg/ml |
| Specificity | Detects rat PTHrP without cross‑reaction to other proteins |
| Shelf life | 6 months |
| Storage temperature | -20 °C (long term) or 2‑8 °C (frequent use) |
| Assay plate | 96‑well ELISA plate |
| Standard material | 2 vials of lyophilized standard |
| Sample diluent volume | 20 ml per kit |
| Wash buffer preparation | 20 ml concentrate, dilute 1:25 with distilled water |
| Stop solution | Sulfuric acid (2 N H2SO4), 10 ml per kit |
The kit can quantify PTHrP concentrations from 3.12 pg/ml up to 200 pg/ml.
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C when used frequently.
A standard ELISA plate reader, a centrifuge, an incubator, Eppendorf tubes, and adjustable pipettes (preferably multichannel) are needed.
Measure the absorbance at 450 nm within 15 minutes after adding the stop solution.