This document introduces the enzyme-linked immunosorbent assay kit for rat pulmonary surfactant-associated protein provided by USCN Life Science Inc. The kit is for in vitro research use only and is used to quantitatively measure the SP-D content in rat serum, plasma, bronchoalveolar lavage fluid, or other related biological fluids.
This manual provides detailed procedures for the rat lung surfactant protein D (SP‑D) ELISA kit (model E1039Ra). It covers sample collection, storage conditions, reagent preparation, assay workflow, plate washing, and data analysis, along with safety warnings and storage guidelines.
The kit uses a 96‑well microplate format, a biotin‑streptavidin detection system, and reads absorbance at 450 nm (±10 nm). The document also includes instructions for preparing standard curves and calculating concentrations from optical density values. This manual is written in Chinese.