This assay is intended for the quantitative determination of HA in rat serum, plasma or other relevant biological fluids.
This manual provides detailed guidance for the USCNK E0182Ra ELISA kit designed to quantify hyaluronic acid in serum, plasma, or other biological fluids. It lists all required reagents, plate format, and preparation steps, and explains the assay principle based on antibody capture and colorimetric detection.
The document describes sample collection, storage conditions, reagent preparation, step‑by‑step incubation and washing procedures, and data analysis methods, including standard curve generation and calculation of sample concentrations. This manual is written in Chinese.
| Lower limit of detection | 12.6 pg/mL |
|---|---|
| Incubation time (substrate development) | 2 hours |