This document provides instructions for the use of the Rat Leptin Receptor (LEPR) ELISA Kit manufactured by Uscn Life Science Inc. Wuhan. The kit is intended for in vitro research use and is used to quantitatively measure the concentration of LEPR in rat serum, plasma, or other related biological fluids.
This manual provides detailed instructions for using the USCNK E0083Ra ELISA kit to quantify rat leptin receptor (LEPR) in biological samples. It lists all reagents supplied, required equipment, and step‑by‑step procedures for plate preparation, sample incubation, detection, and data analysis.
Key points include sample collection and storage guidelines, incubation temperatures, washing protocols, and safety precautions for handling acidic stop solution. The document also specifies the assay’s detection range and limit of detection, ensuring reliable quantitative results. This manual is written in Chinese.
| Detection range | 0.78 ng/mL – 50 ng/mL |
|---|---|
| Limit of detection | 0.45 ng/mL |
| Plate type | 96‑well pre‑coated plate |
| Incubation temperature | 37 °C |
| Sample storage temperature | -20 °C or -80 °C |
The kit can detect LEPR concentrations from 0.78 ng/mL up to 50 ng/mL.
After centrifugation, serum should be stored at –20 °C or –80 °C until analysis.
Incubate the coated plate at 37 °C for 2 hours before adding detection reagents.
The stop solution contains sulfuric acid; avoid contact with skin, eyes, clothing, and handle with care.