This document introduces the Rat CNP ELISA Kit produced by USCN Life Science Inc. The kit is designed for in vitro research and is used to quantitatively measure the content of CNP in rat serum, plasma, or other related biological fluids.
This manual describes the principle of the double‑antibody sandwich ELISA for rat C‑type natriuretic peptide (CNP) and lists all reagents supplied with the kit as well as equipment that must be prepared by the user. It includes detailed instructions for sample collection, storage, reagent preparation, assay steps, plate washing, and data analysis.
Safety precautions, storage conditions, shelf‑life information, and troubleshooting tips are also provided to ensure reliable results. The kit requires a microplate reader with a 450 ± 10 nm filter and samples should be kept at –20 °C to maintain stability. This manual is written in Chinese.
| Detection range | 15.6 pg/mL – 1000 pg/mL |
|---|---|
| Lower limit of detection | 4.2 pg/mL |
| Required plate reader wavelength | 450 ± 10 nm |
| Shelf life | 6 months |
The kit can detect CNP concentrations from 15.6 pg/mL up to 1000 pg/mL.
A microplate reader with a 450 ± 10 nm filter is required.
The kit is valid for six months from the date of receipt.