This kit is used to quantify C-myc in mouse tissue homogenate or related biological fluid.
About this manual
This manual provides detailed guidance for using the USCNK E1290Ra rat C‑myc ELISA kit. It describes the kit components, preparation of samples, the ELISA principle, and step‑by‑step assay procedures, including incubation times and plate handling.
Additional sections cover data analysis, calculation of concentrations, storage conditions for reagents and plates, and safety warnings. The document is intended for laboratory personnel performing quantitative C‑myc measurements in biological samples. This manual is written in Chinese.
What's inside
- Reagents and Materials
- Assay Principle
- Sample Preparation
- Assay Procedure
- Data Analysis
- Storage and Stability
- Safety Precautions
Specifications
| Detection range | 0.312 ng/mL – 20 ng/mL |
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| Minimum detection limit | 0.16 ng/mL |
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| Measurement wavelength | 450 nm |
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| Plate type | 96‑well plate |
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| Incubation temperature | 37 °C |
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| Storage temperature for reagents | -20 °C (or -80 °C for long term) |
Frequently asked questions
What is the detection range of the rat C‑myc ELISA kit?
The kit can detect C‑myc concentrations from 0.312 ng/mL up to 20 ng/mL.
At what wavelength should the absorbance be measured?
Absorbance should be measured at 450 nm.
How should the kit reagents be stored?
Reagents should be sealed and stored at –20 °C (or –80 °C for longer periods).
What type of plate is used in the assay?
The assay uses a 96‑well microplate.