This kit is used for the quantitative determination of ANG in rat serum, plasma or other relevant biological fluids.
This manual describes the USCNK E1254Ra ELISA kit, which uses a 96‑well plate and a sandwich ELISA format to determine ANG levels in rat blood vessel samples. It lists all components, including coated plates, standards, detection reagents, TMB substrate and washing solutions, and provides safety and storage instructions.
The procedure covers sample collection, reagent preparation, incubation steps at 37 °C, washing cycles, color development with TMB, and reading absorbance at 450 nm. Data analysis instructions for generating a standard curve (0.312‑20 ng/mL) and calculating sample concentrations are included, along with guidelines for reagent storage at –20 °C. This manual is written in Chinese.
| Detection wavelength | 450 nm |
|---|---|
| Filter wavelength | 450 ± 10 nm |
| Detection limit | 0.076 ng/mL |
| Standard curve range | 0.312 ng/mL – 20 ng/mL |
| Plate type | 96‑well plate |
| Incubation time | 2 h (primary) / 1 h (secondary) |
| Reagent storage temperature | -20 °C |
The absorbance is measured at 450 nm.
All reagents, including standards and detection solutions, should be stored at –20 °C.
The assay can detect ANG concentrations down to 0.076 ng/mL.