This document provides instructions for the use and expected applications of the Rat Creatine Kinase (CKM) Enzyme-Linked Immunosorbent Assay Kit produced by Uscn Life Science Inc. Wuhan.
This manual provides a complete overview of the USCNK rat muscle‑type creatine kinase (CKM) ELISA kit, including a list of all reagents, required equipment, and safety warnings. It explains the assay principle, sample preparation, and step‑by‑step procedures for plate coating, incubation, washing, and detection.
The document also details storage conditions for the kit components, recommended incubation temperatures, and data analysis guidelines, ensuring reliable quantitative results for CKM measurement in serum, plasma, or other biological fluids. This manual is written in Japanese.
| Plate type | 96‑well microplate |
|---|---|
| Incubation temperature | 37 °C |
| Detection wavelength | 450 nm |
| Detection limit | 1 UL |
| Assay range (OD) | 0.001–3.000 OD |
The lowest detectable amount is 1 UL.
Measure absorbance at 450 nm.