This enzyme-linked immunosorbent assay kit is used to quantitatively determine the level of a2-PI in rat serum, plasma or other related biological fluids by double antibody sandwich ELISA.
This manual provides detailed guidance for the USCNK E0160Ra ELISA kit designed to quantitatively measure rat α2‑Plasmin inhibitor (a2‑PI) in serum, plasma, or other biological fluids. It lists the kit components, explains the assay principle, and describes sample handling and preparation steps.
The document also covers reagent preparation, step‑by‑step plate handling, washing procedures, and data analysis, along with storage conditions for reagents and samples, safety warnings, and troubleshooting tips. This manual is written in Chinese.
| Detection Range | 7.8 ng/mL – 500 ng/mL |
|---|---|
| Minimum Detection Limit | 1.85 ng/mL |
The kit can detect a2‑PI concentrations from 7.8 ng/mL up to 500 ng/mL.
Yes, the kit uses sulfuric acid in the stop solution, which is corrosive; users should avoid contact with skin, eyes, and clothing.