This ELISA kit is used for quantitative determination of TPO in rat serum, plasma or other biological fluids by using a double antibody sandwich ELISA.
The USCNK E0135Ra kit provides a 96‑well ELISA for quantitative measurement of rat thrombopoietin (TPO) in serum, plasma or tissue lysates. The kit includes coated microplates, standards, detection antibodies, HRP conjugate, TMB substrate and stop solution.
The manual describes reagent handling, storage conditions, sample preparation, the step‑by‑step assay protocol (including incubation at 37 °C), washing procedures, and how to read absorbance at 450 nm. It also includes calculations for generating a standard curve and troubleshooting tips. This manual is written in Japanese.
| Detection range | 7.8 pg/mL – 500 pg/mL |
|---|---|
| Limit of detection | 3.6 pg/mL |
The lowest detectable concentration is 3.6 pg/mL.