This document is the user manual for the Rat Placenta Growth Factor (PLGF) ELISA Kit by Uscn Life Science Inc. Wuhan. The kit is used for quantitative measurement of PLGF levels in rat serum, plasma, or other related biological fluids. The kit includes a range of reagents and equipment, as well as instructions for specimen collection and storage. The storage and expiration dates of the kit are also provided. The assay principle is based on the sandwich ELISA method, where PLGF antibodies are immobilized on a microplate, and the bound PLGF is detected and visualized using biotinylated antibodies and HRP-labeled avidin. The colorimetric reaction is measured using a spectrophotometer at 450nm to calculate the sample concentration.
This manual provides detailed instructions for the USCNK E0114Ra rat Placental Growth Factor (PLGF) ELISA kit. It covers sample handling, reagent preparation, assay procedure, plate washing, and result calculation.
Safety precautions, storage conditions, and troubleshooting tips are also included to ensure reliable performance and accurate quantification of PLGF in biological samples. This manual is written in Chinese.
| Plate format | 96-well |
|---|---|
| Detection range | 7.8 pg/mL – 500 pg/mL |
The kit can quantify PLGF from 7.8 pg/mL up to 500 pg/mL.
Store the plate at –20 °C with a desiccant, avoiding moisture.
Avoid contact with skin, eyes, and clothing because it contains sulfuric acid.