This document provides instructions for using the Rat Follicle-Stimulating Hormone (FSH) Enzyme-Linked Immunosorbent Assay (ELISA) Kit, which is intended for in vitro research use only. The kit is used to quantitatively measure FSH levels in rat serum, plasma, or other related biological fluids.
This manual describes the components of the rat Follicle‑stimulating hormone (FSH) ELISA kit, the assay principle, and the required reagents. It explains how to collect and store serum or plasma samples, prepare standards, and perform the ELISA procedure on a 96‑well plate.
Detailed washing steps, incubation times, and data analysis instructions are included, along with storage conditions for reagents and the kit itself. Safety precautions and troubleshooting tips are also provided. This manual is written in Chinese.
| Assay format | 96‑well plate |
|---|---|
| Detection wavelength | 450 nm |
| Detection range | 0.001‑3.000 OD |
| Minimum detection limit | 0.29 mlU/mL |
| Reagent storage temperature | -20 °C |
The assay is read at 450 nm.
The kit uses a 96‑well microplate.
The lowest detectable concentration is 0.29 mlU/mL.