This kit is used to quantitatively measure the Fbg content in rat serum, plasma, cell culture supernatant or other related liquids.
This manual describes the ELISA method for quantitative measurement of rat fibrinogen (Fbg) in serum, plasma, or other liquid samples. It outlines the composition of the kit, including coated antibodies, biotin‑labeled detection antibodies, HRP conjugate, and TMB substrate, and explains how color development correlates with Fbg concentration.
The document provides detailed procedures for reagent preparation, sample handling, incubation steps, washing, and calculation of concentrations. It also includes storage guidelines for samples and reagents, safety notes, and troubleshooting tips. This manual is written in Chinese.
| Detection range | 1.36 µg/mL – 100 µg/mL |
|---|---|
| Measurement wavelength | 450 nm |
The kit can quantify fibrinogen concentrations from 1.36 µg/mL up to 100 µg/mL.
Absorbance should be measured at 450 nm after stopping the reaction.