This document describes the features and usage of the Rat Elastin (ELN) Enzyme-Linked Immunosorbent Assay (ELISA) Kit produced by USCN Life Science Inc.
This manual describes the ELISA kit for detecting rat elastic protein (ELN). It lists all supplied reagents, including a 96‑well plate, standard solutions, detection solutions A and B, TMB substrate, and washing buffers, and explains the principle of the sandwich ELISA using biotin‑labeled antibodies and HRP‑conjugated streptavidin.
Detailed procedures cover sample preparation, plate coating, incubation steps, washing, substrate development, and data analysis. The document also includes storage conditions for reagents, safety warnings, and troubleshooting tips. This manual is written in Chinese.
| Detection range | 1.56 ng/mL – 100 ng/mL |
|---|---|
| Minimum detection limit | 0.78 ng/mL |
| Plate type | 96‑well plate |
The kit can detect ELN concentrations from 1.56 ng/mL up to 100 ng/mL.
Standard solutions, detection solutions A and B, and the 96‑well plate should be stored at –20 °C; sealed plates with desiccant are also kept at –20 °C.
The minimum detectable ELN concentration is 0.78 ng/mL.