This document provides the instructions for using the Rat IFNγ Elisa Kit produced by USCN Life Science Inc. The kit is intended for in vitro research use and is used to quantitatively measure the IFNγ content in rat serum, plasma, or other related biological fluids.
This manual describes the USCN E0049R ELISA kit for quantitative detection of rat IFN‑γ in serum, plasma or other biological fluids. It includes the principle of the double‑antibody sandwich ELISA, the list of reagents supplied, and the equipment required for the assay.
The document provides detailed procedures for sample collection, reagent preparation, plate loading, incubation, washing, substrate development, and data analysis, as well as storage conditions, detection limits, and safety warnings. This manual is written in Chinese.
| Detection range | 15.6 pg/mL – 1000 pg/mL |
|---|---|
| Lowest detection limit | 3.8 pg/mL |
| Plate type | 96‑well pre‑coated plate |
| Recommended microplate reader filter | 450 ± 10 nm |
| Storage temperature | -20 °C |
All reagents should be stored at –20 °C, and the sealed plate should be kept with desiccant at the same temperature.
Read the absorbance at 450 nm (±10 nm).
The kit is valid for six months after opening, provided it is stored at –20 °C and protected from moisture.