This kit is used for quantitative detection of LEP in rat serum, plasma, cell culture supernatant or other related biological fluids.
This manual describes the ELISA kit designed for quantitative measurement of rat leptin (LEP) in biological fluids such as serum, plasma, and cell‑culture supernatants. It includes the assay principle, required reagents, sample handling, and step‑by‑step procedures for performing the test.
The kit contains a 96‑well assay plate, standards, sample diluent, biotin‑labeled antibody, HRP‑avidin, substrate solution, wash buffer, and stop solution. Detailed instructions cover sample collection, storage conditions, preparation of reagents, plate washing, incubation times, and data analysis using absorbance at 450 nm. This manual is written in Chinese.
| Detection range | 0.156 ng/ml – 10 ng/ml |
|---|---|
| Limit of detection | 0.039 ng/ml |
| Shelf life | 6 months |
| Storage temperature (long term) | -20 °C |
| Storage temperature (frequent use) | 2–8 °C |
The kit can detect leptin concentrations from 0.156 ng/ml up to 10 ng/ml.
Store the kit at -20 °C for long‑term storage, or at 2–8 °C when used frequently.
The kit is valid for 6 months from the date of manufacture.