E0685Hu ELISA kit is used for the quantitative determination of CD14 in human serum, plasma or other related biological fluids.
This manual provides detailed instructions for the USCNK E0685Hu human CD14 ELISA kit. It describes the principle of the sandwich ELISA, lists all reagents and consumables, and explains how to collect and store serum, plasma, or other biological samples before testing.
The document guides users through the assay procedure, plate washing, incubation conditions, and data analysis, and includes safety warnings, storage recommendations, and troubleshooting tips to ensure reliable results. This manual is written in Chinese.
| Detection range | 0.94 ng/mL – 60 ng/mL |
|---|---|
| Minimum detection limit | 0.23 ng/mL |
| Incubation temperature | 18‑25 °C |
| Reagent storage temperature | -20 °C (some reagents at -80 °C) |
The kit can detect CD14 concentrations from 0.94 ng/mL up to 60 ng/mL.
The minimum detectable concentration is 0.23 ng/mL.
Reagents should be stored at –20 °C (some at –80 °C) and kept sealed to avoid moisture.