α-EP enzyme-linked immunosorbent assay kit instruction manual, used for quantitative measurement of α-EP content in human serum, plasma or other related liquids
This manual describes the principle, reagents, sample handling, and step‑by‑step procedure for the Human α‑EP ELISA immunoassay kit. It explains how the double‑antibody sandwich format is used, how to prepare standards and samples, and how to perform the enzymatic color development and measurement.
The document also provides guidance on washing, plate handling, storage conditions for reagents, safety notes, and calculation of results, including the detection range and how to handle high‑concentration samples. This manual is written in Chinese.
| Detection range | 1.56 ng/mL – 100 ng/mL |
|---|---|
| Standard concentration | 100 ng/mL (stock) |
| Storage temperature (reagents) | -20 °C or 2‑8 °C depending on reagent |
| Shelf life | 6 months |
The kit can detect α‑EP concentrations from 1.56 ng/mL up to 100 ng/mL.
Some reagents should be stored at –20 °C, while others are kept at 2‑8 °C as indicated on the label.
The kit has a shelf life of six months.
The kit is intended for human serum, plasma, or other related liquids.