This document provides instructions for using the Rat S100B Protein ELISA Kit. The kit is designed for quantitative measurement of S100B protein in rat serum, plasma, cell culture supernatant, or other related biological fluids using the ELISA method. It has high specificity and a low detection limit.
This manual describes the components of the rat S100B ELISA immunoassay kit and provides detailed instructions for preparing reagents, collecting and storing samples, and performing the assay. It also lists equipment that must be supplied by the user, such as a plate reader and centrifuge.
The document explains the dilution principles for standards, biotin‑antibody, and HRP‑avidin reagents, outlines the step‑by‑step assay procedure, and gives guidance on calculation of sample concentrations and important precautions to ensure reliable results. This manual is written in Chinese.
| Detection range | 312 pg/ml – 20000 pg/ml |
|---|---|
| Minimum detection limit | 78 pg/ml |
| Specificity | No cross‑reaction with other related proteins |
| Shelf life | 6 months |
| Storage temperature | -20 °C (long‑term) or 2‑8 °C (short‑term use) |
The assay can quantify S100B concentrations from 312 pg/ml up to 20 000 pg/ml.
The lowest detectable concentration is 78 pg/ml.
Store the kit at –20 °C for long‑term storage, or at 2‑8 °C for short‑term use.
A standard ELISA plate reader, high‑speed centrifuge, incubator, clean tubes (including Eppendorf tubes), adjustable pipettes with tips, and distilled water are needed but not provided.