IL-35 ELISA Kit Instruction Manual, for use in ELISA to quantitatively measure IL-35 in human serum, plasma or other relevant fluids.
This manual describes the intended application of the IL-35 ELISA kit for quantitative measurement of human IL-35 in serum, plasma, or other fluids using a sandwich ELISA format. It lists all kit components, reagent preparation steps, and storage conditions.
Detailed step‑by‑step procedures cover plate coating, sample addition, incubation times, washing, substrate development, and termination. The document also provides guidance on sample collection, handling, and precautions to ensure accurate results. This manual is written in Chinese.
| Detection range | 15.6 pg/ml – 1,000 pg/ml |
|---|---|
| Standard concentration | 1.000 pg/ml |
| Incubation temperature | 37°C |
| Incubation time for substrate | 30 minutes (protected from light) |
The kit can detect IL-35 concentrations from 15.6 pg/ml up to 1,000 pg/ml.
The substrate solution should be kept protected from light and used within 30 minutes after adding to the plate at 37 °C.
Incubations are performed at 37 °C for the specified times.