Rat Melanocyte Antibody (MC Ab ) Enzyme-linked Immunoassay Kit Instructions for Use, This kit is for in vitro research use only. The expected application is to quantitatively measure the MC Ab content in rat serum, plasma, cell culture supernatant or other relevant biological fluids by ELISA.
The MC Ab ELISA immunoassay kit is designed for quantitative measurement of rat MC antibodies in various biological samples. The kit includes a 96‑well coated plate, lyophilized standards, sample diluent, detection reagents, substrate and stop solution, together with detailed instructions for reagent preparation and sample handling.
The procedure involves adding standards or samples to the coated wells, followed by biotinylated detection antibody, HRP‑streptavidin, washing steps, substrate development, and measurement of absorbance at 450 nm. Proper washing, incubation times, and temperature control are critical for accurate results, and the manual outlines storage conditions and safety precautions for all reagents. This manual is written in Chinese.
| Plate format | 96‑well |
|---|---|
| Standard concentration | 1000 U/ml |
| Detection solution A volume | 1 × 120 µl per kit |
| Detection solution B volume | 1 × 120 µl per kit |
| Substrate solution volume | 1 × 10 ml per kit |
| Washing buffer volume | 1 × 30 ml per kit (diluted 25‑fold before use) |
| Stop solution | 2 N H₂SO₄, 1 × 10 ml per kit |
| Reagent storage temperature | 2‑8 °C or –20 °C depending on reagent |
The kit can quantify MC antibodies in rat serum, plasma, cell‑culture supernatant, or other related biological fluids.
Incubate the plate at 37 °C in the dark for up to 30 minutes before adding the stop solution.