This kit can simultaneously detect natural or recombinant rat PEDF, and has no cross-reaction with other related proteins.
This manual provides detailed guidance for the rat pigment epithelium‑derived factor (PEDF) ELISA kit. It covers kit components, storage conditions, sample collection, preparation of reagents, step‑by‑step assay procedures, and calculation of results.
The document also includes practical tips such as plate washing methods, precautions to avoid false positives, and recommended equipment needed for the assay. Users are advised to follow the standard curve for each run and to store reagents according to the specified temperatures. This manual is written in Chinese.
| Shelf life | 6 months |
|---|---|
| Storage temperature | -20°C long term, 2‑8°C short term |
| Assay plate type | 96‑well plate |
| Detection wavelength | 450 nm |
| Standard stock concentration | 2000 ng/ml |
Store the kit at –20 °C for long‑term storage or at 2‑8 °C for short‑term use.
Measure the absorbance at 450 nm using a plate reader.
The assay uses a 96‑well plate.