This document introduces the usage and expected applications of the Chicken Adiponectin (ADP) ELISA Kit produced by USCN Life Science Inc. The kit is intended for in vitro research use and is used to quantitatively measure the levels of ADP in chicken serum, plasma, or other biological fluids. The experimental principle involves the use of a sandwich ELISA method with solid-phase carrier and labeled antibodies to measure the concentration of ADP.
This manual describes the ELISA kit for detecting chicken fat adiponectin (ADP). It lists all supplied reagents, required equipment, and storage conditions, and explains the assay principle based on antibody binding and color development with TMB substrate.
Detailed procedures cover sample preparation (serum, blood, or other biological fluids), plate loading, incubation steps at 37 °C, washing, addition of detection reagents, and measurement of absorbance at 450 nm. Data analysis instructions include generating a standard curve and calculating sample concentrations. This manual is written in Chinese.
| Detection range | 1.56 ng/mL – 100 ng/mL |
|---|---|
| Minimum detection limit | 0.8 ng/mL |
| Measurement wavelength | 450 nm (±10 nm filter) |
| Plate type | 96‑well plate |