Rat Norepinephrine (NA) Enzyme Linked Immunosorbent Assay (ELISA) Kit User Manual, for the quantitative measurement of norepinephrine content in rat serum, plasma, cell culture supernatant or other related biological fluids.
This manual provides detailed guidance for the rat norepinephrine (NA) ELISA immunoassay kit. It describes the composition of the kit, storage conditions for reagents, and the recommended procedures for sample collection, preparation, and assay execution. The protocol includes steps for adding standards, samples, biotin‑labeled antibody, HRP‑avidin, substrate, and stop solution, as well as plate washing and incubation conditions.
Additional sections cover calculation of sample concentrations from the standard curve, important precautions to avoid false results, and tips for equipment setup such as plate readers and centrifuges. The document also lists required ancillary equipment and supplies that are not provided with the kit. This manual is written in Chinese.
| Detection range | 4.7 pg/mL – 300 pg/mL |
|---|---|
| Lowest detection limit | 2 pg/mL |
| Shelf life | 6 months |
| Long‑term storage temperature | -20 °C |
| Short‑term storage temperature | 2 °C – 8 °C |
The kit can detect norepinephrine concentrations from 4.7 pg/mL up to 300 pg/mL.
Reagents should be stored at –20 °C for long‑term storage and at 2 °C–8 °C for frequent use.
The assay’s lowest detection limit is 2 pg/mL.
The kit has a shelf life of 6 months.