This document provides instructions for using the Rat Telomerase (TE) Enzyme-linked Immunosorbent Assay (ELISA) kit, including the experimental principle, composition and preparation of the kit, specimen collection and preservation, and operating procedures.
This manual describes the intended application of the rat telomerase ELISA kit, the composition of the kit, and the required equipment. It provides detailed procedures for sample preparation, reagent preparation, and the ELISA workflow, including incubation times, washing steps, and detection.
Additional sections cover plate washing methods, calculation of sample concentrations using a standard curve, storage conditions for reagents, and safety precautions. The document also includes troubleshooting tips and notes on the stability of the kit components. This manual is written in Chinese.
| Standard concentration | 50 ng/ml |
|---|---|
| Detection wavelength | 450 nm |
| Shelf life | 6 months |
| Reagent storage temperature | -20°C for some reagents, 2‑8°C for others |
Read the absorbance at 450 nm.
The kit has a shelf life of 6 months.
The standard solution is supplied at 50 ng/ml.
Some reagents should be stored at –20 °C, while others should be kept at 2‑8 °C.