This kit is used to quantitatively measure parathyroid hormone (PTH) in rat serum, plasma or other related liquids
About this manual
The kit is intended for quantitative ELISA measurement of rat parathyroid hormone (PTH) in serum, plasma, cell culture supernatants, or other related liquids. It uses a 96‑well plate coated with purified capture antibody, followed by addition of sample or standard, biotinylated anti‑PTH antibody, HRP‑streptavidin, washing steps, and TMB substrate development.
The manual details the kit components, reagent preparation, sample collection and storage, step‑by‑step assay procedure, plate‑washing methods, calculation of concentrations from the standard curve, and important precautions such as avoiding strong light, proper storage temperatures, and preventing incomplete washing. This manual is written in Chinese.
What's inside
- Intended Application
- Kit Components and Reagent Preparation
- Sample Collection and Storage
- Assay Procedure
- Plate Washing Methods
- Calculation
- Precautions
- Detection Range
- General Information
Specifications
| Detection Range | 23.44 pg/ml – 1.500 pg/ml |
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| Lowest Detection Limit | 5.86 pg/ml |
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| Assay Format | 96‑well plate |
Frequently asked questions
What types of samples can be measured with this kit?
The kit can measure rat serum, plasma, or cell culture supernatants for PTH content.
What is the detection range of the assay?
The assay detects PTH concentrations from 23.44 pg/ml up to 1.500 pg/ml.
What is the lowest detectable concentration?
The lowest detection limit is 5.86 pg/ml.