Rat PTH ELISA Kit User Manual

This kit is used to quantitatively measure parathyroid hormone (PTH) in rat serum, plasma or other related liquids


Rat PTH ELISA Kit User Manual - cover page
Brand
rat甲状旁腺激素(PTH)酶联免疫分析试剂盒
Category
Drugs
Document type
User Manual
Language
Chinese
Pages
5
File format
PDF
File size
114 KB
Published
07 January, 2012
Updated
30 September, 2023
MD5 checksum
522D1D258B093653FB94A00FDDBC5025

About this manual

The kit is intended for quantitative ELISA measurement of rat parathyroid hormone (PTH) in serum, plasma, cell culture supernatants, or other related liquids. It uses a 96‑well plate coated with purified capture antibody, followed by addition of sample or standard, biotinylated anti‑PTH antibody, HRP‑streptavidin, washing steps, and TMB substrate development.

The manual details the kit components, reagent preparation, sample collection and storage, step‑by‑step assay procedure, plate‑washing methods, calculation of concentrations from the standard curve, and important precautions such as avoiding strong light, proper storage temperatures, and preventing incomplete washing. This manual is written in Chinese.

What's inside

  • Intended Application
  • Kit Components and Reagent Preparation
  • Sample Collection and Storage
  • Assay Procedure
  • Plate Washing Methods
  • Calculation
  • Precautions
  • Detection Range
  • General Information

Specifications

Detection Range23.44 pg/ml – 1.500 pg/ml
Lowest Detection Limit5.86 pg/ml
Assay Format96‑well plate

Frequently asked questions

What types of samples can be measured with this kit?

The kit can measure rat serum, plasma, or cell culture supernatants for PTH content.

What is the detection range of the assay?

The assay detects PTH concentrations from 23.44 pg/ml up to 1.500 pg/ml.

What is the lowest detectable concentration?

The lowest detection limit is 5.86 pg/ml.

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