ELISA Kit E0310h User Manual

This document describes the usage and principle of the enzyme-linked immunosorbent assay kit for human β2-glycoprotein 1 antibody IgA/G/M (β2-GP1 IgA/G/M), as well as the composition and preparation of the kit.


ELISA Kit E0310h User Manual - cover page
Brand
酶联免疫吸附测定试剂盒使用说明书
Category
Drugs
Document type
User Manual
Language
Chinese
Pages
4
File format
PDF
File size
127 KB
Published
01 January, 2012
Updated
29 September, 2023
MD5 checksum
7769091E106FC0A220CE8A8BA776AE16

About this manual

This manual describes the principle of the ELISA assay, the composition of the kit, and detailed procedures for sample preparation, reagent handling, and plate incubation. It also provides guidance on washing, substrate development, and termination steps, as well as instructions for reading absorbance at 450 nm.

Additional sections cover sample collection and storage, recommended equipment, washing methods (manual and automated), specificity, calculation of results, detection range, limit of detection, storage conditions, and shelf life. Safety notes and troubleshooting tips are included to ensure reliable results. This manual is written in Chinese.

What's inside

  • 实验原理
  • 试剂盒组成及试剂配制
  • 自备物品
  • 标本的采集及保存
  • 操作步骤
  • 洗板方法
  • 特异性
  • 计算
  • 检测范围
  • 最低检测限

Specifications

Product numberE0310h
Detection range1.56 UL – 100 UL
Lowest detection limit0.78 UL
Shelf life6 months
Storage temperature (short‑term)4 °C
Storage temperature (long‑term)-20 °C

Frequently asked questions

What is the detection range of the ELISA kit?

The kit can detect concentrations from 1.56 UL up to 100 UL.

How should the kit be stored?

Store the reagents at -20 °C for long‑term storage; short‑term storage can be at 4 °C, and keep the plate sealed with desiccant after opening.

What is the minimum detection limit?

The lowest detectable amount is 0.78 UL.

How long is the kit valid after receipt?

The kit has a shelf life of six months.

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