This document describes the usage and principle of the enzyme-linked immunosorbent assay kit for human β2-glycoprotein 1 antibody IgA/G/M (β2-GP1 IgA/G/M), as well as the composition and preparation of the kit.
About this manual
This manual describes the principle of the ELISA assay, the composition of the kit, and detailed procedures for sample preparation, reagent handling, and plate incubation. It also provides guidance on washing, substrate development, and termination steps, as well as instructions for reading absorbance at 450 nm.
Additional sections cover sample collection and storage, recommended equipment, washing methods (manual and automated), specificity, calculation of results, detection range, limit of detection, storage conditions, and shelf life. Safety notes and troubleshooting tips are included to ensure reliable results. This manual is written in Chinese.
What's inside
- 实验原理
- 试剂盒组成及试剂配制
- 自备物品
- 标本的采集及保存
- 操作步骤
- 洗板方法
- 特异性
- 计算
- 检测范围
- 最低检测限
Specifications
| Product number | E0310h |
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| Detection range | 1.56 UL – 100 UL |
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| Lowest detection limit | 0.78 UL |
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| Shelf life | 6 months |
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| Storage temperature (short‑term) | 4 °C |
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| Storage temperature (long‑term) | -20 °C |
Frequently asked questions
What is the detection range of the ELISA kit?
The kit can detect concentrations from 1.56 UL up to 100 UL.
How should the kit be stored?
Store the reagents at -20 °C for long‑term storage; short‑term storage can be at 4 °C, and keep the plate sealed with desiccant after opening.
What is the minimum detection limit?
The lowest detectable amount is 0.78 UL.
How long is the kit valid after receipt?
The kit has a shelf life of six months.