eiaab E0177Ch ELISA Immunoassay Kit Manual

E0177Ch chicken α-Glu ELISA kit is used for quantitative detection of α-Glu in chicken serum, plasma, cell culture supernatant, tissue homogenate or other related biological fluids.


eiaab E0177Ch ELISA Immunoassay Kit Manual - cover page
Brand
鸡α葡萄糖苷酶(a-Glu)酶联免疫分析试剂盒
Category
Drugs
Document type
User Manual
Language
Chinese
Pages
4
File format
PDF
File size
126 KB
Published
01 January, 2012
Updated
30 September, 2023
MD5 checksum
D4F5F2D7BF88540ED4F77048B12C7496

About this manual

The eiaab E0177Ch ELISA immunoassay kit provides all reagents needed to measure chicken α‑glucosidase (a‑Glu) in various biological samples. The kit includes a 96‑well coated plate, standard proteins, sample diluent, detection reagents, substrate, stop solution and detailed instructions.

The protocol covers sample preparation, reagent reconstitution, step‑by‑step incubation, washing, substrate development, and data analysis using a microplate reader at 450 nm. Proper handling, storage conditions, and safety precautions are also outlined to ensure reliable results. This manual is written in Chinese.

What's inside

  • Principle of ELISA
  • Reagents and Preparation
  • Sample Collection and Storage
  • Assay Procedure
  • Incubation and Washing
  • Substrate Development and Stop Reaction
  • Data Analysis
  • Safety and Storage

Specifications

Detection range1.56 U/L - 100 U/L
Lowest detection limit0.78 U/L
Shelf life6 months
Long‑term storage temperature-20 °C
Plate format96‑well plate

Frequently asked questions

What types of samples can be tested with this kit?

The kit can quantify a‑Glu in chicken serum, plasma, cell‑culture supernatant, tissue homogenate and other related biological fluids.

How should the reagents be prepared before use?

All reagents must be brought to room temperature before use; they should not be dissolved directly at 37 °C.

What is the detection limit of the kit?

The lowest detectable concentration is 0.78 U/L.

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