E0177Ch chicken α-Glu ELISA kit is used for quantitative detection of α-Glu in chicken serum, plasma, cell culture supernatant, tissue homogenate or other related biological fluids.
About this manual
The eiaab E0177Ch ELISA immunoassay kit provides all reagents needed to measure chicken α‑glucosidase (a‑Glu) in various biological samples. The kit includes a 96‑well coated plate, standard proteins, sample diluent, detection reagents, substrate, stop solution and detailed instructions.
The protocol covers sample preparation, reagent reconstitution, step‑by‑step incubation, washing, substrate development, and data analysis using a microplate reader at 450 nm. Proper handling, storage conditions, and safety precautions are also outlined to ensure reliable results. This manual is written in Chinese.
What's inside
- Principle of ELISA
- Reagents and Preparation
- Sample Collection and Storage
- Assay Procedure
- Incubation and Washing
- Substrate Development and Stop Reaction
- Data Analysis
- Safety and Storage
Specifications
| Detection range | 1.56 U/L - 100 U/L |
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| Lowest detection limit | 0.78 U/L |
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| Shelf life | 6 months |
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| Long‑term storage temperature | -20 °C |
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| Plate format | 96‑well plate |
Frequently asked questions
What types of samples can be tested with this kit?
The kit can quantify a‑Glu in chicken serum, plasma, cell‑culture supernatant, tissue homogenate and other related biological fluids.
How should the reagents be prepared before use?
All reagents must be brought to room temperature before use; they should not be dissolved directly at 37 °C.
What is the detection limit of the kit?
The lowest detectable concentration is 0.78 U/L.