E0927r Rat Histamine (HIS) ELISA Kit is a quantitative ELISA kit for the determination of HIS in rat serum, plasma or other relevant biological fluids.
This manual describes the intended application, assay principle, kit components, and detailed step‑by‑step procedures for the rat histamine (HIS) ELISA kit. It includes guidance on sample collection, reagent preparation, plate washing, data analysis, and storage of reagents.
The kit is intended for in‑vitro research only and provides a detection range of 0.312–20 ng/mL with a limit of detection of 0.078 ng/mL. Proper handling, temperature control, and avoidance of cross‑contamination are emphasized to ensure reliable results. This manual is written in Chinese.
| Product Number | E0927r |
|---|---|
| Detection Range | 0.312 ng/mL – 20 ng/mL |
| Limit of Detection | 0.078 ng/mL |
| Plate Type | 96‑well ELISA plate |
Serum, plasma, or other related biological fluids can be used for measuring rat histamine levels.
Standard and detection solutions should be stored at –20 °C; other reagents can be kept short‑term at 4 °C and long‑term at –20 °C.
Incubate the plate at 37 °C for 15–30 minutes in the dark before adding the stop solution.