This document is a user manual of PAPP-A ELISA kit, introducing the kit components, operation procedures and result interpretation.
This manual describes the DRG PAPP‑A ELISA kit (model EIA‑2397) used for quantitative detection of pregnancy‑associated plasma protein‑A (PAPP‑A) in human serum or plasma. It includes an introduction to the test principle, a complete list of kit components, sample handling guidelines, step‑by‑step assay procedures, and data analysis methods.
Safety precautions, storage requirements, assay performance characteristics, and legal compliance information are also provided to ensure reliable results in clinical laboratories. This manual is written in Chinese.
| Assay dynamic range | 0 – 30 µg/mL |
|---|---|
| Analytical sensitivity | < 0.133 µg/mL (serum) |
| Storage temperature (unopened) | 2 – 8 °C |
| Sample types | Serum or plasma (EDTA, heparin, citrate) |
Serum or plasma (EDTA, heparin or citrate anticoagulated) may be used for the assay.
The kit uses a sandwich ELISA: micro‑well plates are coated with a monoclonal anti‑PAPP‑A antibody, samples bind, then a horseradish peroxidase‑conjugated antibody forms a sandwich complex whose colour development is proportional to PAPP‑A concentration.