T4 DNA Ligase is an enzyme that can catalyze the connection of DNA molecules. It can catalyze the connection of DNA with sticky ends or smooth ends, as well as the connection of DNA with RNA and a few RNA.
The Takara T4 DNA Ligase is an enzyme that catalyzes the joining of DNA fragments, including blunt and cohesive ends, and can also ligate DNA to RNA. The manual lists the enzyme activity as 350 U/µl (25,000 Units per vial) and defines 1 U as 0.008 Weiss Unit.
The leaflet details the recommended storage at –20 °C, the composition of the 10× ligase buffer (Tris‑HCl, KCl, DTT, EDTA, glycerol), and provides a step‑by‑step example for ligation reactions, including buffer preparation and reaction conditions. This manual is written in Japanese.
| Enzyme activity | 350 U/µl (25,000 Units per vial) |
|---|---|
| 10× T4 DNA Ligase Buffer composition | Tris‑HCl (pH 7.5) 10 mM, KCl 50 mM, DTT 1 mM, EDTA 0.1 mM, Glycerol 50 % |
| Unit definition | 1 U = 0.008 Weiss Unit |
The buffer contains Tris‑HCl (pH 7.6) 660 mM, MgCl₂ 66 mM, DTT 100 mM, and ATP 1 mM.
Each vial contains 350 U/µl, totaling 25,000 Units of T4 DNA Ligase.