Key facts
- Manuals
- 1
- Languages
- JA
- Last added
- 2011-12-16
Featured T4 manuals
Takara T4 DNA Ligase Product Leaflet
The Takara T4 DNA Ligase is an enzyme that catalyzes the joining of DNA fragments, including blunt and cohesive ends, and can also ligate DNA to RNA. The manual lists the enzyme activity as 350 U/µl (25,000 Units per vial) and defines 1 U as 0.008 Weiss Unit.
The leaflet details the recommended storage at –20 °C, the composition of the 10× ligase buffer (Tris‑HCl, KCl, DTT, EDTA, glycerol), and provides a step‑by‑step example for ligation reactions, including buffer preparation and reaction conditions. This manual is written in Japanese.
Covers: Applications · Precautions · Buffer Composition · Usage Example · Storage Conditions · Package Contents
Latest T4 manuals
Takara T4 DNA Ligase Product Leaflet
Product Leaflet 2 pages · 180 KB · JATakara T4 DNA Ligase product leaflet provides enzyme activity, storage conditions, buffer composition, and usage instructions.
Questions answered in T4 manuals
What is the composition of the 10× T4 DNA Ligase Buffer?
The buffer contains Tris‑HCl (pH 7.6) 660 mM, MgCl₂ 66 mM, DTT 100 mM, and ATP 1 mM.
What enzyme activity is provided in the vial?
Each vial contains 350 U/µl, totaling 25,000 Units of T4 DNA Ligase.