This document is the instruction manual for THUNDERBIRD SYBR qPCR Mix, a quantitative PCR reagent kit manufactured by TOYOBO CO.,LTD. The kit is intended for research use only and should not be used for diagnostic or clinical purposes. The user is advised to follow general laboratory precautions and safety procedures. Additionally, the purchase of this product includes a limited license for internal research use.
The Thunderbird SYBR qPCR Mix is a 2× premixed reagent for SYBR Green real‑time PCR. It contains reaction buffer, SYBR Green dye, dNTPs, Mg²⁺, Taq DNA polymerase and an antibody‑based hot‑start system, enabling simplified preparation and high reproducibility.
The manual details product composition, recommended storage conditions, preparation of the reaction mix, PCR cycling parameters, primer design guidelines, and compatibility with both block‑type and glass‑capillary real‑time PCR instruments. It also provides a list of related products and a FAQ section. This manual is written in Chinese.